Retinol-Binding Protein 4 Actives Adipocyte Renin–Angiotensin System via Toll-Like Receptor 4

Abstract Background To investigate the relationship between levels of serum adipokine retinol-binding protein 4 (RBP4) and essential hypertension as well to explore role mechanism RBP4-induced activation renin–angiotensin system (RAS) in adipocyte. Methods From December 2016 March 2019, 96 hypertensive patients without treatment 64 healthy nonhypertensive subjects were recruited. The difference RBP4 2 groups was compared, analyzed by multivariate logistic regression. In mouse primary white adipocytes, effect on RAS adipocyte determined experiment adenovirus transfected overexpression. Protein expression Toll-like receptor (TLR4) key components western blotting. level angiotensin II (Ang II) cell supernatant measured enzyme-linked immunosorbent assay. We also observed TLR4 inhibitor TAK242 induced Results Serum significantly higher with than [30.14 (23.76–40.65) vs. 25.05 (20.05–31.04) µg/ml, P < 0.01]. Multivariate regression analysis showed that independently associated [OR 1.10 (95% CI 1.06–1.14), = 0.002]. RBP4-transfected angiotensin-converting enzyme, angiotensinogen, renin/angiotensin-forming enzyme 1, Ang (P 0.05) increased. Whereas, markedly reduced when treated 0.05). Conclusions is hypertension. TLR4-mediated might be mechanisms underlying effects blood pressure.